Ganoderma microsporum immunomodulatory protein acts as a multifunctional broad-spectrum antiviral against SARS-CoV-2 by interfering virus binding to the host cells and spike-mediated cell fusionArticle Published on 2022-11-012022-11-15 Journal: Biomedicine & pharmacotherapy = Biomédecine & phar [Category] SARS, 변종, 치료제, [키워드] ACE2 ACE2-expressing cells acute respiratory syndrome alleviate Antiviral binding blocked Broad-spectrum antiviral against SARS-CoV-2 caused Cell Cell fusion cell lines cell-free virus cells characteristic coronavirus Delta determine Diseases downregulation Effect evaluated examined flow cytometry fluorescence microscopy fluorescent Fungal immunomodulatory proteins fusion GMI HEK293T cell host cell immunomodulatory immunoprecipitation in vitro Infection Infectivity ingredient inhibit inhibited inhibitor Interaction investigated less mechanism multifunctional omicron Protein pseudotyped virus reported SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pseudovirus SARS-CoV-2 pseudovirus SARS-CoV-2 pseudovirus infection SARS-CoV-2 spike protein SARS-CoV-2 variant spike mutation spike variant spike variants Strains Viral binding. virus were used [DOI] 10.1016/j.biopha.2022.113766 PMC 바로가기
Photochemical Identification of Auxiliary Severe Acute Respiratory Syndrome Coronavirus 2 Host Entry Factors Using μMapArticle Published on 2022-09-142022-11-16 Journal: Journal of the American Chemical Society [Category] SARS, 변종, [키워드] ACE2 ACE2 expression acute respiratory syndrome auxiliary cell entry complex coronavirus coronavirus 2 COVID-19 pandemic Critical Delta eight engage ENhance enzyme expression facilitate Factor Genetic Host human cell identification identify identifying labeling lymphocyte membrane fusion pathophysiology platform powerful tool Protein receptor receptors respiratory SARS-CoV-2 significantly spike variant systemically the spike protein tissues transmembrane serine protease viral entry Wuhan [DOI] 10.1021/jacs.2c06806 PMC 바로가기
High-throughput drug screening allowed identification of entry inhibitors specifically targeting different routes of SARS-CoV-2 Delta and Omicron/BA.1고용량 약물 스크리닝을 통해 SARS-CoV-2 Delta 및 Omicron/BA.1의 다양한 경로를 구체적으로 표적으로 하는 진입 억제제 식별 가능Article Published on 2022-07-012022-09-11 Journal: Biomedicine & pharmacotherapy = Biomédecine & phar [Category] COVID19(2023년), SARS, 변종, 신약개발, 유전자 메커니즘, [키워드] acute respiratory syndrome coronavirus Alpha antagonist antagonists applied BA.1 Beta Caco2 cells Cathepsin-L cell entry cells cellular entry chlorpromazine Combination Compound D614G Delta drug Drug repurposing Drug screening Entry inhibitor FDA-approved drugs G protein G protein coupled receptor antagonist G-protein help highest identify Infection route inhibit inhibitors lentiviral Lentiviral vector lentiviral vectors mechanism membrane fusion omicron pathway phenothiazine preference pseudotyped pseudovirus pseudovirus pseudoviruses receptor receptor antagonist Receptor binding receptors respiratory responsible resulting SARS-CoV-2 several variant several variants Severe acute respiratory syndrome specific inhibitors spike variant synergistic targets TMPRSS2 variant Variant of concern. viral entry VoC VOCs [DOI] 10.1016/j.biopha.2022.113104 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Variant Exposures Elicit Antibody Responses With Differential Cross-Neutralization of Established and Emerging Strains Including Delta and OmicronSARS-CoV-2 변종 노출은 Delta 및 Omicron을 포함한 기존 및 신규 균주의 차등 교차 중화를 통해 항체 반응을 이끌어냅니다.Article Published on 2022-06-012022-09-11 Journal: The Journal of Infectious Diseases [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antibody antibody escape Antibody Response Antibody responses B.1.1.529 B.1.1.529 (omicron) B.1.617.2 B.1.617.2 (Delta) booster vaccination breadth contribute coronavirus COVID-19 cross-neutralization Delta exposure exposure to immune immune exposure immune response immune responses Including increase individual Infection natural infection neutralization Neutralizing antibody response neutralizing antibody responses omicron phenotype Phenotypes respiratory response SARS-CoV-2 sensitivity sera severe acute respiratory syndrome Coronavirus spike mutation Spike protein spike variant strain Transmission vaccination variant Variant. variants variants of concern [DOI] 10.1093/infdis/jiab635 PMC 바로가기 [Article Type] Article
Antibody Profiling in COVID-19 Patients with Different Severities by Using Spike Variant Protein Microarrays스파이크 변이 단백질 마이크로어레이를 사용한 다양한 중증도의 COVID-19 환자에서 항체 프로파일링Article Published on 2022-05-032022-09-11 Journal: Analytical Chemistry [Category] MERS, 변종, 진단, [키워드] ACE2 ACE2 binding antibody antibody isotypes collected competition COVID-19 COVID-19 patient COVID-19 patients COVID-19 severity Critical critical cases death demonstrated Disease progression dose-dependent inhibition Drug screening elevated evaluated except for healthy subject healthy subjects humoral Humoral immunity immune inhibitors Microarray Mild Neutralizing activity Neutralizing antibodies nonstructural protein 3 patients Perindopril Profiling Protein protein 3 ramipril serum serum IgG severity Specificities specificity spike spike variant spike variants variant [DOI] 10.1021/acs.analchem.1c05567 PMC 바로가기 [Article Type] Article
Rapid SARS-CoV-2 Adaptation to Available Cellular Proteases사용 가능한 세포 프로테아제에 대한 신속한 SARS-CoV-2 적응Article Published on 2022-03-092022-09-11 Journal: Journal of Virology [Category] SARS, 변종, 유전자 메커니즘, [키워드] adaptation Alpha Alpha variant B.1.1.529 B.1.1.7 B.1.617.2 binding cathepsin Cathepsins cleavage clinical isolates conditions Consensus coronavirus coronavirus spike priming deep sequencing delta variant dominant Efficiency Evolution furin furin cleavage motif furin cleavage site Genome genomes genomic Genotype growth heparan sulfate highlight human cell human cells identify independent inhibit isolate isolates lack mechanism minor motif mutated Omicron variant processed protease Proteases Rapid recent S1/S2 site SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants selection pressure selective sequence spike mutation spike mutation. Spike protein spike protein of SARS-CoV-2 spike variant stability subpopulation sulfate Surveillance the spike protein These cells TMPRSS2 variant variants Vero Vero E6 Vero E6 cell VERO E6 cells virus virus growth virus pathogenesis wild type [DOI] 10.1128/jvi.02186-21 PMC 바로가기 [Article Type] Article
Co-circulation of two SARS-CoV-2 variant strains within imported pet hamsters in Hong Kong홍콩에서 수입된 애완용 햄스터 내에서 두 개의 SARS-CoV-2 변종 균주의 공동 순환Coronaviruses Published on 2022-02-272022-09-10 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] adaptive animal animals aviation binding energy can not coronavirus COVID-19 Delta delta variant epidemiologically Europe European hamster hamsters Hong Kong Human human ACE2 interspecies molecular docking Netherland Netherlands outbreak Outbreaks Patient phylogenetically positive Prevent RBD reported respiratory Respiratory Coronavirus risk SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variant SARS-CoV-2 variants severe acute respiratory coronavirus spike mutation spike receptor-binding domain spike variant spike variants strain Strains subsequent suggested susceptible Testing these viruses Transmission triggered variant virus viruses warehouse while zoonosis [DOI] 10.1080/22221751.2022.2040922 PMC 바로가기 [Article Type] Coronaviruses
Conformational Flexibility and Local Frustration in the Functional States of the SARS-CoV-2 Spike B.1.1.7 and B.1.351 Variants: Mutation-Induced Allosteric Modulation Mechanism of Functional Dynamics and Protein StabilitySARS-CoV-2 스파이크 B.1.1.7 및 B.1.351 변이체의 기능 상태에서 구조적 유연성 및 국부적 좌절: 기능 역학 및 단백질 안정성의 돌연변이 유발 알로스테릭 조절 메커니즘Article Published on 2022-01-312022-09-10 Journal: International Journal of Molecular Sciences [Category] SARS, 변종, 유전자 메커니즘, 진단, [키워드] ACE2 host receptor allosteric interactions allosteric regulation Analysis B.1.1.7 B.1.351 B.1.351 variant B.1.351 variants binding characterized conformational conformational change conformational dynamics conformational state D614G driver Dynamics Effect flexibility forms Frustration functional greater host cell host cell receptor hotspot identify inter-protomer interface intrinsic local frustration mechanism modulation molecular Molecular simulation molecular simulations mutant Mutation mutational scanning mutational variants open plasticity Protein protein stability receptor Region regulate Regulation required residue SARS-CoV-2 B.1.1.7 SARS-CoV-2 spike protein SARS-CoV-2 spike proteins spike Spike protein Spike proteins spike variant spike variants stability state suggested the SARS-CoV-2 the spike protein Trajectories trajectory Transmissibility variant virus infectivity [DOI] 10.3390/ijms23031646 PMC 바로가기 [Article Type] Article
A cell-free high throughput assay for assessment of SARS-CoV-2 neutralizing antibodiesSARS-CoV-2 중화 항체 평가를 위한 무세포 고처리량 분석Article Published on 2022-01-252022-09-11 Journal: New biotechnology [Category] MERS, SARS, 변종, 진단, [키워드] ACE2 Analysis angiotensin angiotensin converting enzyme angiotensin converting enzyme 2 antibodies antibody bead Bead-based binding Cell-free Efficiency enzyme handling Immunity individual inhibiting instrument live SARS-CoV-2 measure microneutralization Microneutralization assay neutralization neutralize Neutralizing pandemic performed predict Prevalence produced provide Pseudoneutralization recognizing robust SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 antibody SARS-CoV-2 neutralizing antibody SARS-CoV-2 spike protein SARS-CoV-2 variants SARS-CoV-2. serological serological test Serological tests Seven shown Spike protein spike variant the antibodies the SARS-CoV-2 the spike protein [DOI] 10.1016/j.nbt.2021.10.002 PMC 바로가기 [Article Type] Article
SARS-CoV-2 variants with reduced infectivity and varied sensitivity to the BNT162b2 vaccine are developed during the course of infection감염성이 감소하고 BNT162B2 백신에 대한 감도가 다양한 SARS-COV-2 변이체가 감염 과정에서 개발됩니다.Clinical Trial Published on 2022-01-122022-08-13 Journal: PLoS Pathogens [Category] Biochemistry, 변종, 임상, 치료기술, 치료법, [키워드] addition Affect Analysis analyzed Anti-spike Antibody neutralization antibody neutralization activity Author BNT162b2 BNT162b2 vaccine circulating convalescent plasma Course COVID-19 vaccine COVID-19 vaccines demonstrated elicit employment Evolution Frequency highlight Host human host human population impair in-depth analysis increase in individual infected individual infected individuals Infection infectivity capacity intra-host SARS-CoV-2 variant intra-host SARS-CoV-2 variants Intra-host variant intra-host variants L1197I mutation landscape less low frequencies mRNA mRNA-based mutated mutated variant mutated variants N gene N genes natural infection neutralization Neutralizing antibodies nose-throat swab nose-throat swabs pandemic plasma plasma neutralization activity platform Point mutation potential circulating variants Protective Quasispecies quasispecy raised reduced respiratory Respiratory system S gene S2 subunit SARS-CoV-2 SARS-CoV-2 evolution SARS-CoV-2 intra-host variability SARS-CoV-2 intra-host variant SARS-CoV-2 intra-host variants SARS-CoV-2 mutagenesis SARS-CoV-2 variant SARS-CoV-2 variants sensitivity Sequencing Sequencing database Sequencing databases single-genome sequencing Spike protein Spike proteins spike variant Spread swab sample swab samples the SARS-CoV-2 the spike protein vaccinated individuals vaccination rate variant variants variants of concern [DOI] 10.1371/journal.ppat.1010242 PMC 바로가기 [Article Type] Clinical Trial